Mastitis Research Today is a free monthly online journal that collates and summarizes the latest research about Mastitis, including details on symptoms, treatment, breast feeding.

Association between a case study of asymptomatic ovine listerial mastitis and the contamination of soft cheese and cheese processing environment with Listeria monocytogenes in Portugal.

Pintado CM, Grant KA, Halford-Maw R, Hampton MD, Ferreira MA, McLauchlin J

Escola Superior Agrária, Instituto Politécnico de Castelo Branco, Castelo Branco, Portugal.

For 5 months, the udders of milking ewes, raw ewe's milk, cheese, and the plant and environment of a cheese manufacturer in Portugal were investigated using standard methods for the presence of Listeria spp. An association between subclinical mastitis and Listeria monocytogenes in a single lactating sheep was investigated by visual inspection of udders for signs of inflammation, application of somatic cell counts, the California mastitis test, pH measurement to milk, and culture of L. monocytogenes and Staphylococcus spp. To track the routes of contamination by L. monocytogenes, 103 isolates were characterized by molecular serotyping and amplified fragment length polymorphism, and a selection was further tested by pulsed-field gel electrophoresis. This study provides molecular and epidemiological evidence tracking the persistence of a single L. monocytogenes strain causing a subclinical udder infection without obvious inflammation in a single ewe. This infection was the likely source of contamination of raw milk that was subsequently used to produce unpasteurised milk cheese and resulted in a single strain of this bacterium colonizing the processing environment and the final cheese product.

Published 18 June 2009 in Foodborne Pathog Dis, 6(5): 569-75.
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Articles on Mastitis published 12 June 2009:

Leukocyte populations and cytokine expression in the mammary gland in a mouse model of Streptococcus agalactiae mastitis.   J Med Microbiol, 58: 951-8.

Streptococcus agalactiae is a contagious, mastitis-causing pathogen that is highly adapted to survive in the bovine mammary gland. This study used a BALB/c mouse model of Streptococcus agalactiae mastitis to evaluate leukocyte populations in regional lymph nodes and cytokine expression in the mammary gland involved in the immune response against Streptococcus agalactiae. It was found that the bacteria replicated efficiently in the mammary gland, peaking after 24 h and increasing by 100-fold. ... [Abstract] [Full-text]

Articles on Mastitis published 11 June 2009:

Systemic therapy as a first choice treatment for idiopathic granulomatous mastitis.   J Cutan Pathol, 36(6): 689-91.

Idiopathic granulomatous mastitis clinically and histologically mimics an inflammatory carcinoma. A correct approach including ultrasound, clinical and histological analysis can safely identify a patient with this pathology, orienting to adequate therapy with anti-inflammatory and antibiotic drugs and leaving the surgical approach only for case unresponsive to medical therapy. [Abstract] [Full-text]

Articles on Mastitis published 10 June 2009:

Database of cattle candidate genes and genetic markers for milk production and mastitis.   Anim Genet.

A cattle database of candidate genes and genetic markers for milk production and mastitis has been developed to provide an integrated research tool incorporating different types of information supporting a genomic approach to study lactation, udder development and health. The database contains 943 genes and genetic markers involved in mammary gland development and function, representing candidates for further functional studies. The candidate loci were drawn on a genetic map to reveal ... [Abstract] [Full-text]

Articles on Mastitis published 9 June 2009:

Esculentin 1-21: a linear antimicrobial peptide from frog skin with inhibitory effect on bovine mastitis-causing bacteria.   J Pept Sci.

Mastitis, or inflammation of the mammary gland, is the most common and expensive illness of dairy cows throughout the world. Although stress and physical injuries may give rise to inflammation of the udders, infections by bacteria or other microorganisms remain the major cause, and infusion of antibiotics is the main treatment approach. However, the increased emergence of multidrug-resistant pathogens and the production of milk contaminated with antibiotics has become a serious threat in the ... [Abstract] [Full-text]

Sonographic features of neonatal mastitis and breast abscess.   Pediatr Radiol.

BACKGROUND: Neonatal mastitis and neonatal breast abscess are uncommon. Although well described in the pediatric and surgical literature, there is a paucity of reports describing their sonographic features. OBJECTIVE: To describe and illustrate the sonographic features of neonatal mastitis and neonatal breast abscess. MATERIALS AND METHODS: We reviewed the medical database of a large children's health-care center from 2000 through 2008 for patients presenting in the first 8 weeks of life with ... [Abstract] [Full-text]

Articles on Mastitis published 5 June 2009:

Classification of Inflammatory Breast Disorders and Step by Step Diagnosis.   Breast J.

In this study, the authors proposed a classification of inflammatory breast disorders based on which a practical systematic scheme in diagnosis was applied aiming to differentiate simple forms of mastitis from more complicated and malignant forms. The study population included 197 female patients who were clinically or pathologically diagnosed as having mastitis. All patients underwent Ultrasound examination. Mammography was performed for 133/197 cases. Cases of simple mastitis and periductal ... [Abstract] [Full-text]

Effect of endotoxic mastitis on epithelial cell numbers in the milk of dairy cows.   Am J Vet Res, 70(6): 796-9.

Objective-To measure epithelial cell percentages and somatic cell counts (SCCs) in milk and determine whether isoflupredone acetate reduces mammary gland epithelial cell sloughing in cows with acute endotoxin-induced mastitis. Animals-13 lactating Holstein cows. Procedures-Determination of SCC and flow cytometric analysis of cytokeratin-positive (epithelial) cells in milk were performed before and 12 hours after induction of mastitis via intramammary administration of bacterial endotoxin in 8 ... [Abstract] [Full-text]

Articles on Mastitis published 2 June 2009:

Comparative genomics of Toll-like receptor signalling in five species.   BMC Genomics, 10: 216.

BACKGROUND: Over the last decade, several studies have identified quantitative trait loci (QTL) affecting variation of immune related traits in mammals. Recent studies in humans and mice suggest that part of this variation may be caused by polymorphisms in genes involved in Toll-like receptor (TLR) signalling. In this project, we used a comparative approach to investigate the importance of TLR-related genes in comparison with other immunologically relevant genes for resistance traits in five ... [Abstract] [Full-text]

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